| 產(chǎn)品編號(hào) | bs-1074R-BF488 |
| 英文名稱 | Rabbit Anti-Nrf2/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的核因子2相關(guān)因子2抗體 |
| 別 名 | Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號(hào) 合成與降解 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nrf2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） 核細(xì)胞系因子2相關(guān)因子Nrf2，是一種對(duì)氧化還原反應(yīng)敏感的轉(zhuǎn)錄因子，可以通過(guò)正向促進(jìn)基因的表達(dá)，來(lái)轉(zhuǎn)錄翻譯產(chǎn)生一些抗氧化物，行使著異化解毒的酶類和藥物的高效外排泵作用。 有學(xué)者認(rèn)為：核因子2相關(guān)因子2蛋白在保護(hù)細(xì)胞免受環(huán)境毒性物質(zhì)影響方面發(fā)揮著極其重要的作用，它指導(dǎo)某些基因促進(jìn)細(xì)胞的防御工事，同時(shí)也刺激某些重要解毒酶對(duì)毒性物質(zhì)，如黃曲霉素的解毒和排毒作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation:Nrf2Antibody, conjugated (bs-1074R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation:Nrf2Antibody, conjugated (bs-1074R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation:Nrf2Antibody, conjugated (bs-1074R-BF488) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
The Hela ( treated with MG-132 （10 μM，10 h）) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-Nrf2 antibody (bs-1074R-BF488): 1 μg/10^6 cells. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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