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Rabbit Anti-VAPA/BF488 Conjugated antibody (bs-4226R-BF488)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產(chǎn)品編號 bs-4226R-BF488
英文名稱 Rabbit Anti-VAPA/BF488 Conjugated antibody
中文名稱 BF488標記的囊泡相關(guān)膜蛋白相關(guān)蛋白A抗體
別    名 VAMP A; VAMP associated protein A; VAP 33; VAP A; VAP33; Vesicle associated membrane protein associated protein A; VAPA_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細胞生物  免疫學  信號轉(zhuǎn)導  細胞粘附分子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,  (predicted: Mouse, Rat, Dog, Cow, Rabbit, )
產(chǎn)品應用 Flow-Cyt=2ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 28kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human VAPA
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Two transcript variants encoding distinct isoforms have been identified.

Function:
May play a role in vesicle trafficking.

Subunit:
Homodimer, and heterodimer with VAPB. Interacts with VAMP1, VAMP2, STX1A, BET1, SEC22C and with the C-terminal domain of OCLN. Interacts with OSBPL1A. Interacts (via MSP domain) with ZFYVE27; may retain ZFYVE27 in the endoplasmic reticulum and regulate its function in cell projections formation.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type IV membrane protein.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. Contains 1 MSP domain.

Database links:
UniProtKB/Swiss-Prot: Q9P0L0.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片
Blank control:A431. Primary Antibody (green line): Rabbit Anti-VAPA antibody (bs-4226R-BF488) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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