| 產(chǎn)品編號(hào) | bsm-2079M-BF488 |
| 英文名稱1 | Mouse Anti-Cecropin/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的殺菌肽/天蠶抗菌肽單克隆抗體 |
| 別 名 | attacin. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 1D8 |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 4kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Hyalophora cecropia |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: No data available. Function: Cecropins have lytic and antibacterial activity against several Gram-positive and Gram-negative bacteria. Subcellular Location: Secreted. Post-translational modifications: A protein with the same sequence as cecropin A, but lacking the carboxyl blocking group, has been isolated and called cecropin C (PubMed:7140755). Similarity: Belongs to the cecropin family. Database links: GenBank: AAP93872.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 天蠶素(cecropins)是第一個(gè)被發(fā)現(xiàn)的動(dòng)物抗菌肽，1980年，由Boman等從天蠶蛹中分離得到。該類多肽抗生素一般含有37～39個(gè)氨基酸殘基，不含半胱氨酸，其N端區(qū)域具有強(qiáng)堿性，可形成近乎完美的雙親螺旋結(jié)構(gòu)，而在C端區(qū)域可形成疏水螺旋，兩者之間有甘氨酸和脯氨酸形成的鉸鏈區(qū)，多數(shù)多肽的C端被酰胺化，酰胺化對(duì)其抗菌活性具有重要作用。天蠶素(cecropins)對(duì)革蘭氏陽性菌、部分革蘭氏陰性菌具有很強(qiáng)的殺傷力，而對(duì)真菌和真核細(xì)胞沒有毒性。目前研究認(rèn)為，抗菌肽是最具潛力的抗生素替代品之一。而天蠶素又是目前研究比較清楚的抗菌肽。 天蠶素抗菌肽利用N端所帶的正電荷與原核細(xì)胞的細(xì)胞膜磷脂雙分子層密集的負(fù)電荷靜電相吸，C端柔性的插入疏水區(qū)，在兩親性α-螺旋作用下，在原核細(xì)胞膜上形成4nm的離子通道，離子通道的形成改變了細(xì)胞膜內(nèi)外的滲透壓，細(xì)胞膜內(nèi)物質(zhì)大量外滲，致細(xì)菌細(xì)胞死亡，起到殺菌作用。天蠶素抗菌肽對(duì)細(xì)菌、病毒、念珠菌、原蟲、癌細(xì)胞具有廣譜的活性，能抑制革蘭氏陽性和陰性球菌和桿菌，如綠膿桿菌、大腸桿菌、傷寒桿菌、痢疾桿菌、布氏桿菌、金黃色葡萄球菌、溶血性鏈球菌等等，并對(duì)感染植物的多種病原菌也有明顯的殺菌作用。另外，天蠶素抗菌肽對(duì)高度耐藥的傷寒桿菌、耐藥性金葡菌同樣敏感，對(duì)感冒、疤疹病毒能抑制其繁殖，對(duì)瘧原蟲、痢疾阿米巴均有效 |
